• Product NameCyclin-L1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Cyclin-L1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Cyclin-L1.
• Target NameCyclin-L1
• Alternative Names    Cyclin-L; CCNL1;
• Accession No. Swiss-Prot: Q9UK58
  NCBI Gene ID: 57018
• UniprotQ9UK58
• Gene ID57018;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Transcriptional regulator which participates in regulating the pre-mRNA splicing process. Seems to be involved in the regulation of RNA polymerase II (pol II). Functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. May be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). Inhibited by the CDK-specific inhibitor p21.

A. Hamid Boulares, J. Biol. Chem., Aug 1999; 274: 22932.
Alessandra Pagano, Am J Physiol Lung Cell Mol Physiol, Jun 2007; 10.1152.
Mi Young Kim, Genes & Dev., Sep 2005; 19: 1951 - 1967.
Silke S¨¹sse, Nucleic Acids Res., Feb 2004; 32: 669 - 680.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin-L1 antibody #33465.
• Western blot analysis of extracts from HepG2 cells, using Cyclin-L1 antibody #33465.
• Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using Cyclin-L1 antiobdy #33465. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting