• Product NameBCL-XL(Ab-62) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total BCL-XL protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.60~64 (A-D-S-P-A) derived from human BCL-XL .
• Target NameBCL-XL
• Alternative Names    Apoptosis regulator Bcl-X
• Accession No. Swiss-Prot: Q07817
  NCBI Protein: NP_612815.1
• UniprotQ07817
• Gene ID598;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 30kd
  Western blotting: 1:500~1:1000
  

Potent inhibitor of cell death. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases By similarity. Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.

Basu A, et.al.( 2003) Mar 13; 538(1-3): 41-7
Cheng E.H.-Y. et.al. (1996) Nature 379:554-556.
Sattler M. et.al. (1997), Science 275:983-986.
Muchmore S.W.et.al (1996).Nature 381:335-341
Yu J., Zhang L. Mol. (2001)Cell 7:673-682

• Western blot analysis of extracts from 293 and Hela cells using BCL-XL(Ab-62) antibody #21061.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting