• Product NamePGP9.5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total PGP9.5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human PGP9.
• Target NamePGP9.5
• Alternative Names    UCH-L1; neuron cytoplasmic protein 9.5; ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thiolesterase L1;
• Accession No. Swiss-Prot: P09936
  NCBI Gene ID: 7345
• UniprotP09936
• Gene ID7345;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Diana L. Mandelker, Cancer Res., Jun 2005; 65: 4963 - 4968.
Taiji Yamazaki, Clin. Cancer Res., Jan 2002; 8: 192.
Ekmel Tezel, Clin. Cancer Res., Dec 2000; 6: 4764.
Kenji Hibi, Am. J. Pathol., Sep 1999; 155: 711.

• Immunohistochemical analysis of paraffin-embedded human brain tissue using PGP9.5 antibody #33472.
• Western blot analysis of extracts from 293 cells, using PGP9.5 antibody #33472.
• Western blot analysis of extracts from 293 cells (Lane 2), using PGP9.5 antiobdy #33472. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting