• Product NameRIT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RIT1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human RIT1.
• Target NameRIT1
• Alternative Names    GTP-binding protein Rit1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1; RIBB; RIT
• Accession No. Swiss-Prot: Q92963
  NCBI Gene ID: 6016
• UniprotQ92963
• Gene ID6016;
• SDS-PAGE MW 25kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation.

Manabu Furukawa, Mol. Cell. Biol., Nov 2000; 20: 8185 - 8197.
Angus Chen, J. Biol. Chem., May 2000; 275: 15432.
Kenneth Wu, Mol. Cell. Biol., Feb 2000; 20: 1382 - 1393.
Kenneth Wu, J. Biol. Chem., Jan 2002; 277: 516 - 527.

• Western blot analysis of extracts from Jurkat cells, using RIT1 antibody #33482.
• Western blot analysis of extracts from K562 cells (Lane 2), JK cells (Lane 3) and 293 cells (Lane 4), using RIT1 antiobdy #33482. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting