• Product NameStefin B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total stefin B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human stefin B.
• Target NameStefin B
• Alternative Names    CPI-B; CST6; CYTB; EPM1; PME
• Accession No. Swiss-Prot: P04080
  NCBI Gene ID: 1476
• UniprotP04080
• Gene ID1476;
• SDS-PAGE MW 11kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

Janko Kos, Clin. Cancer Res., Feb 2000; 6: 505.
Gregor Anderluh, FEBS J., Jun 2005; 272: 3042 - 3051.
Sabina Rabzelj, Protein Sci., Oct 2005; 14: 2713 - 2722.
Manca Kenig, Protein Sci., Jan 2004; 13: 63.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stefin B antibody #33490.
• Immunofluorescence analysis of A549 cells, using Stefin B antibody #33490.
• Western blot analysis of extracts from A549 cells, using Stefin B antibody #33490.
• Western blot analysis of extracts from A549 cells using Stefin B antibody #33490. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting