• Product NameSynuclein β Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Synuclein β protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Synuclein β antibody.
• Target NameSynuclein β
• Alternative Names    SYUB; beta-synuclein;
• Accession No. Swiss-Prot: Q16143
  NCBI Gene ID: 6620
• UniprotQ16143
• Gene ID6620;
• SDS-PAGE MW 14kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

Yuxin Fan, Hum. Mol. Genet., Oct 2006; 15: 3002 - 3011.
Makoto Hashimoto, J. Biol. Chem., May 2004; 279: 23622 - 23629.
Cristine Alves da Costa, J. Biol. Chem., Sep 2003; 278: 37330 - 37335.
Heather Snyder, J. Biol. Chem., Mar 2005; 280: 7562 - 7569.

• Immunohistochemical analysis of paraffin-embedded human brain tissue using Synuclein β antibody #33494.
• Immunofluorescence analysis of A549 cells, using Synuclein β antibody #33494.
• Western blot analysis of extracts from HeLa cells, using Synuclein β antibody #33494.
• Western blot analysis of extracts from 293 cells (Lane 2) and cos-7 cells (Lane 3), using Synuclein β antiobdy #33494. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting