• Product NameTIMP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total TIMP1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TIMP1.
• Target NameTIMP1
• Alternative Names    tissue inhibitor of metalloproteinase 1;
• Accession No. Swiss-Prot: P01033
  NCBI Gene ID: 7076
• UniprotP01033
• Gene ID7076;
• SDS-PAGE MW 24kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

Anieta M. Sieuwerts, Clin. Chem., May 2007; 10.1373.
KM Fong, Clin. Cancer Res., Aug 1996; 2: 1369.
E. Susol, Rheumatology, Dec 2000; 39: 1332 - 1336.
Christopher J Jackson, Ann Rheum Dis, Mar 1998; 57: 158 - 161.

• Western blot analysis of extracts from 293 cells, using TIMP1 antibody #33502.
• Western blot analysis of extracts from cos-7 cells (Lane 2), using TIMP1 antiobdy #33502. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting