• Product NameTIMP3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total TIMP3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TIMP3.
• Target NameTIMP3
• Alternative Names    Metalloproteinase inhibitor 3 [Precursor]; TIMP-3; Tissue inhibitor of metalloproteinases 3; Protein MIG-5; TIMP3
• Accession No. Swiss-Prot: P35625
  NCBI Gene ID: 7078
• UniprotP35625
• Gene ID7078;
• SDS-PAGE MW 25kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Uria J.A., Cancer Res. 54:2091-2094(1994).
Wilde C.G., DNA Cell Biol. 13:711-718(1994).
Silbiger S.M., Gene 141:293-297(1994).

• Western blot analysis of extracts from HUVEC cells, using TIMP3 antibody #33504.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting