• Product NameAOS1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total AOS1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human AOS1.
• Target NameAOS1
• Alternative Names    AOS1; HSPC140; SAE1; SUA1; SUMO-1 activating enzyme E1 N subunit
• Accession No. Swiss-Prot: Q9UBE0
  NCBI Gene ID: 10055
• UniprotQ9UBE0
• Gene ID10055;
• SDS-PAGE MW 38kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.
Walter Makous, Invest. Ophthalmol. Vis. Sci., Sep 2006; 47: 4160 - 4167.
Hsin-Pei Shih, J. Cell Sci., Mar 2002; 115: 1259.
Thomas Buschmann, J. Biol. Chem., Oct 2001; 276: 40389 - 40395.

• Immunofluorescence analysis of HuvEc cells, using AOS1 antibody #33506.
• Western blot analysis of extracts from 293 cells, using AOS1 antibody #33506.
• Western blot analysis of extracts from Colo cells, HepG2 cells and 293 cells using AOS1 antibody #33506. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting