• Product NameFAS Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Fas protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human Fas.
• Target NameFAS
• Alternative Names    Apo-1; CD95; FasL receptor; TNFRSF6; TNR6
• Accession No. Swiss-Prot: P25445
  NCBI Gene ID: 355
• UniprotP25445
• Gene ID355;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Michael J. Pinkoski, Am JPhysiol Gastrointest Liver Physiol, Mar 2000; 278: 354.
Morgan Jenkins, J. Immunol., Aug 1999; 163: 1195.
Nicholas Mitsiades, J Natl Cancer Inst, Oct 1999; 91: 1678 - 1684.
JOS? E. CARDIER, FASEB J, Nov 1999; 13: 1950.

• Western blot analysis of extracts from LOVO cells, using Fas antibody #33510.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Fas antibody #33510.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting