• Product NameNCoR1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NCoR1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human NCoR1.
• Target NameNCoR1
• Alternative Names    KIAA1047; N-CoR1;
• Accession No. Swiss-Prot: O75376
  NCBI Gene ID: 9611
• UniprotO75376
• Gene ID9611;
• SDS-PAGE MW 270kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6.

Igor Girault, Clin. Cancer Res., Apr 2003; 9: 1259.
Xinjian Peng, Carcinogenesis, May 2007; 28: 1000 - 1007.
Satoshi Mikawa, Genome Res., May 2007; 17: 586 - 593.
Claire M. Banwell, Clin. Cancer Res., Apr 2006; 12: 2004 - 2013.

• Western blot analysis of extracts from MDA-MB-435 cells, using NCoR1 antibody #33512.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NCoR1 antibody #33512.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting