• Product NameNYREN18 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NYREN18 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human NYREN18.
• Target NameNYREN18
• Alternative Names    NEDD8 ultimate buster-1; NUB1; NY-REN-18 antigen;
• Accession No. Swiss-Prot: Q9Y5A7
  NCBI Gene ID: 51667
• UniprotQ9Y5A7
• Gene ID51667;
• SDS-PAGE MW 70kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.

Sarah M. Mense, Toxicol. Sci., Sep 2006; 93: 125 - 135.
Gunter Schmidtke, J. Biol. Chem., Jul 2006; 281: 20045 - 20054.s
Kunikazu Tanji, Am. J. Pathol., Aug 2006; 169: 553 - 565.
Miguel Ramalho-Santos, Science, Oct 2002; 298: 597.

• Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using NYREN18 antibody #33514.
• Western blot analysis of extracts from COS7 cells, using NYREN18 antibody #33514.
• Western blot analysis of extracts from HuvEc cells (Lane 2) and JK cells (Lane 3), using NYREN18 antiobdy #33514. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting