• Product NamePIAS3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total PIAS3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human PIAS3.
• Target NamePIAS3
• Alternative Names    PROTEIN INHIBITOR OF ACTIVATED STAT3; protein inhibitor of activated STAT 3;
• Accession No. Swiss-Prot: Q9Y6X2
  NCBI Gene ID: 10401
• UniprotQ9Y6X2
• Gene ID10401;
• SDS-PAGE MW 68kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth.

Jiang-Hong Man, Nucleic Acids Res., Nov 2006; 34: 5552 - 5566.
Amir Sonnenblick, Mol. Cell. Biol., Dec 2004; 24: 10584 - 10592.
Jianyin Long, PNAS, Jan 2004; 101: 99.
Hyun Duk Jang, J. Biol. Chem., Jun 2004; 279: 24873 - 24880.

• Western blot analysis of extracts from 293 cells, treated with UV (5mins), using PIAS3 antibody #33517.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PIAS3 antibody #33517.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting