• Product NameSENP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SENP1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human SENP1.
• Target NameSENP1
• Alternative Names    SUMO1; sentrin specific protease 1; EC 3.4.22.-; Sentrin/SUMO-specific protease SENP1;
• Accession No. Swiss-Prot: Q9P0U3
  NCBI Gene ID: 29843
• UniprotQ9P0U3
• Gene ID29843;
• SDS-PAGE MW 73kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity.

Taihei Yamaguchi, Mol. Cell. Biol., Jun 2005; 25: 5171 - 5182.
Jinke Cheng, Mol. Cell. Biol., Jul 2004; 24: 6021 - 6028.
Molly A. Brewer, AACR Meeting Abstracts, Mar 2004; 2004: 1006.
Imke M. Veltman, Hum. Mol. Genet., Jul 2005; 14: 1955 - 1963.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using SENP1 antibody #33519.
• Western blot analysis of extracts from Jurkat cells, using SENP1 antibody #33519.
• Western blot analysis of extracts from JK cells (Lane 2), COS7 cells (Lane 3) and HuvEc cells (Lane 4), using SENP1 antiobdy #33519. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting