• Product NameSENP5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total SENP5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human SENP5.
• Target NameSENP5
• Alternative Names    EC 3.4.22.-; SUMO1/sentrin specific peptidase 5;
• Accession No. Swiss-Prot: Q96HI0
  NCBI Gene ID: 205564
• UniprotQ96HI0
• Gene ID205564;
• SDS-PAGE MW 86kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.

Alessandra Di Bacco, Mol. Cell. Biol., Jun 2006; 26: 4489 - 4498.
Thomas Colby, Plant Physiology, Sep 2006; 142: 318 - 332.
Pei-Zheng Zheng, PNAS, May 2005; 102: 7653 - 7658.
Rodolfo Zunino, J. Cell Sci., Apr 2007; 120: 1178 - 1188.

• Western blot analysis of extracts from COS7 cells, using SENP5 antibody #33521.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using SENP5 antibody #33521.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting