• Product NameSENP8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total SENP8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human SENP8.
• Target NameSENP8
• Alternative Names    DEN1; NEDP; DENEDDYLASE 1; NEDD8-SPECIFIC PROTEASE 1; SENTRIN-SPECIFIC PROTEASE FAMILY
• Accession No. Swiss-Prot: Q96LD8
  NCBI Gene ID: 123228
• UniprotQ96LD8
• Gene ID123228;
• SDS-PAGE MW 27kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.

Tudeviin Gan-Erdene, J. Biol. Chem., Aug 2003; 278: 28892 - 28900.
Debaditya Mukhopadhyay, J. Cell Biol., Sep 2006; 174: 939 - 949.
Thomas Colby, Plant Physiology, Sep 2006; 142: 318 - 332.
Joris Hemelaar, Mol. Cell. Biol., Jan 2004; 24: 84 - 95.

• Western blot analysis of extracts from HuvEc cells, using SENP8 antibody #33524.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using SENP8 antibody #33524.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting