• Product NameTIMP4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total TIMP4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human TIMP4.
• Target NameTIMP4
• Alternative Names    TIMP metallopeptidase inhibitor 4; tissue inhibitor of metalloproteinase 4; Metalloproteinase inhibitor 4;
• Accession No. Swiss-Prot: Q99727
  NCBI Gene ID: 7079
• UniprotQ99727
• Gene ID7079;
• SDS-PAGE MW 26kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.

Jennifer Adjodha, AACR Meeting Abstracts, Apr 2005; 2005: 699.
Kiyoshi Asada, AACR Meeting Abstracts, Apr 2005; 2005: 202.
Elaine K Green, J. Med. Genet., Aug 2000; 37: 581.
Jennifer Adjodha, AACR Meeting Abstracts, Apr 2006; 2006: 1028.

• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TIMP4 antibody #33526.
• Western blot analysis of extracts from NIH/3T3 cells, using TIMP4 antibody #33526.
• Western blot analysis of extracts from HepG2 cells (Lane 2), using TIMP4 antiobdy #33526. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting