• Product NameTopoisomerase II β Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total topoisomerase II β protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human topoisomerase II β.
• Target NameTopoisomerase II β
• Alternative Names    DNA topoisomerase II; beta isozyme; EC 5.99.1.3; TP2B; TOP2B
• Accession No. Swiss-Prot: Q02880
  NCBI Gene ID: 7155
• UniprotQ02880
• Gene ID7155;
• SDS-PAGE MW 183kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Ren¨¦ M. Linka, Nucleic Acids Res., May 2007; 10.1093.
John R. Jenkins, Nucleic Acids Res., Nov 1992; 20: 5587 - 5592.
Kathryn L. Gilroy, Nucleic Acids Res., Mar 2006; 34: 1597 - 1607.
Sophie Dereuddre, Cancer Res., Oct 1997; 57: 4301 - 4308.

• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Topoisomerase II β antibody #33528.
• Western blot analysis of extracts from Jurkat cells, using Topoisomerase II β antibody #33528.
• Western blot analysis of extracts from K562 cells (Lane 2), using TOP2B antiobdy #33528. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting