• Product NameTRADD Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TRADD protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human TRADD.
• Target NameTRADD
• Alternative Names    TNFRSF1A-associated via death domain; Tumor necrosis factor receptor type 1-associated DEATH domain protein; TNFR1-associated DEATH domain protein;
• Accession No. Swiss-Prot: Q15628
  NCBI Gene ID: 8717
• UniprotQ15628
• Gene ID8717;
• SDS-PAGE MW 34kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARFof CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARFof CDKN2A By similarity. Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.

Hiroyasu Inada, J. Cell Biol., Oct 2001; 155: 415.
Kenneth M. Izumi, Mol. Cell. Biol., Aug 1999; 19: 5759 - 5767.
Susumu Rokudai, Mol. Cell. Biol., Dec 2002; 22: 8695 - 8708.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TRADD antibody #33529.
• Western blot analysis of extracts from COS7 cells, using TRADD antibody #33529.
• Western blot analysis of extracts from COS7 cells (Lane 2) and HuvEc cells (Lane 3), using TRADD antiobdy #33529. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting