• Product NameTubulin α Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Tubulin α protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human Tubulin α.
• Target NameTubulin α
• Alternative Names    Alpha-tubulin 1; TBA1; TUBA1; Testis-specific alpha- tubulin; Tubulin H2-alpha
• Accession No. Swiss-Prot: Q71U36
  NCBI Gene ID: 7846/10376
• UniprotQ71U36
• Gene ID7846;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Zhenfeng Zhang et al. Human Bocavirus NP1
Inhibits IFN-β Production by Blocking
Association of IFN Regulatory Factor 3 with
IFNB Promoter(2012) .The Journal of Immunology.
WB Tubulin α Antibody (C0379).
Qun Liu et al. The Cytotoxicity Mechanism
of 6

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Tubulin α antibody #33531.
• Immunofluorescence analysis of NIH/3T3 cells, using Tubulin α antibody #33531.
• Western blot analysis of extracts from MCF7,COLO and HepG2 cells, using Tubulin α antibody #33531.
• Western blot analysis of extracts from 3T3 cells (Lane 2), colo cells (Lane 3) and HuvEc cells (Lane 4), using Tubulin α antiobdy #33531. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting