• Product NameTyrosinase Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total tyrosinase protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human tyrosinase.
• Target NameTyrosinase
• Alternative Names    TYR; EC 1.14.18.1; Monophenol monooxygenase; Tumor rejection antigen AB; SK29-AB
• Accession No. Swiss-Prot: P14679
  NCBI Gene ID: 7299
• UniprotP14679
• Gene ID7299;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.

Patricia Giraldo, Nucleic Acids Res., Nov 2003; 31: 6290 - 6305.
R Halaban, J. Cell Biol., Aug 1983; 97: 480.
Hee-Young Park, J. Biol. Chem., Jun 1999; 274: 16470.
BB Fuller, J. Biol. Chem., Mar 1987; 262: 4024 - 4033.

• Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Tyrosinase antibody #33534.
• Western blot analysis of extracts from HepG2 cells (Lane 2), using Tyrosinase antiobdy #33534. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting