• Product NameUBE1L Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total UBE1L protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human UBE1L.
• Target NameUBE1L
• Alternative Names    D8; UBE2; ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-like; ubiquitin-activating enzyme E1-related protein
• Accession No. Swiss-Prot: P41226
  NCBI Gene ID: 7318
• UniprotP41226
• Gene ID7318;
• SDS-PAGE MW 112kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

Sutisak Kitareewan, PNAS, Mar 2002; 99: 3806.
Keun Il Kim, Mol. Cell. Biol., Jan 2006; 26: 472 - 479.
Ian Pitha-Rowe, Cancer Res., Nov 2004; 64: 8109 - 8115.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBE1L antibody #33536.
• Western blot analysis of extracts from HeLa cells, using UBE1L antibody #33536.
• Western blot analysis of extracts from JK cells (Lane 2), using UBE1L antiobdy #33536. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting