• Product NameUSF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total USF-2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human USF-2.
• Target NameUSF2
• Alternative Names    FIP; upstream transcription factor 2; c-fos interacting;
• Accession No. Swiss-Prot: Q15853
  NCBI Gene ID: 7392
• UniprotQ15853
• Gene ID7392;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Nobuto Shibata, J. Gerontol. A Biol. Sci. Med. Sci., Jul 2006; 61: 660 - 662.
J L Meier, Mol. Cell. Biol., Oct 1994; 14: 6896 - 6906.
Hassan Sellak, J. Biol. Chem., May 2005; 280: 18425 - 18433.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using USF2 antibody #33539.
• Western blot analysis of extracts from HeLa cells, using USF2 antibody #33539.
• Western blot analysis of extracts from HeLa cells and HepG2 cells using USF2 antibody #33539. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting