• Product NameXPA Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total XPA protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human XPA.
• Target NameXPA
• Alternative Names    DNA-repair protein complementing XP-A cells; XPAC; Xeroderma pigmentosum group A complementing protein;
• Accession No. Swiss-Prot: P23025
  NCBI Gene ID: 7507
• UniprotP23025
• Gene ID7507;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.

Gary W. Daughdrill, Nucleic Acids Res., Jul 2003; 31: 4176 - 4183.
Esther M. Hoogervorst, Carcinogenesis, Mar 2003; 24: 613 - 619.
Karen M. Vasquez, PNAS, Apr 2002; 99: 5848.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using XPA antibody #33544.
• Western blot analysis of extracts from COLO205 cells, using XPA antibody #33544.
• Western blot analysis of extracts from COS7 cells (Lane 2), using XPA antiobdy #33544. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting