• Product NameTNF11 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TNF11 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human TNF11.
• Target NameTNF11
• Alternative Names    Tumor necrosis factor ligand superfamily member 11; Receptor activator of nuclear factor kappa B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE
• Accession No. Swiss-Prot: O14788
  NCBI Gene ID: 8600
• UniprotO14788
• Gene ID8600;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Anderson D.M., Nature 390:175-179(1997).
Lacey D.L., Cell 93:165-176(1998).
Nagai M., Biochem. Biophys. Res. Commun. 269:532-536(2000).

• Western blot analysis of extracts from HuvEc cells, using TNF11 antibody #33569.
• Immunofluorescence analysis of HepG2 cells, using TNF11 antibody #33569.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting