• Product NameEPHB6 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total EPHB6 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human EPHB6.
• Target NameEPHB6
• Alternative Names    EPB6; Ephrin type-B receptor 6 precursor; HEP; Tyrosine-protein kinase-defective receptor EPH-6;
• Accession No. Swiss-Prot: O15197
  NCBI Gene ID: 2051
• UniprotO15197
• Gene ID2051;
• SDS-PAGE MW 119kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.

atsuoka H., Biochem. Biophys. Res. Commun. 235:487-492(1997).
Hillier L.W., Nature 424:157-164(2003).
Zhang Z., Protein Sci. 13:2819-2824(2004).

• Western blot analysis of extracts from HeLa cells, 293 cells and COLO cells, using EPHB6 antibody #33577.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using EPHB6 antibody #33577.
• Immunofluorescence analysis of HeLa cells, using EPHB6 antibody #33577.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting