• Product NameM3K13 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total M3K13 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human M3K13.
• Target NameM3K13
• Alternative Names    Mitogen-activated protein kinase kinase kinase 13; EC 2.7.11.25; Mixed lineage kinase; MLK; Leucine zipper-bearing kinase
• Accession No. Swiss-Prot: O43283
  NCBI Gene ID: 9175
• UniprotO43283
• Gene ID9175;
• SDS-PAGE MW 108kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.

Sakuma H., J. Biol. Chem. 272:28622-28629(1997).
Schultz S.J., Cell Growth Differ. 4:821-830(1993).
Ikeda A., FEBS Lett. 488:190-195(2001).

• Western blot analysis of extracts from HeLa cells, HUVEC cells and HepG2 cells, using M3K13 antibody #33582.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting