• Product NameTNF12 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TNF12 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TNF12.
• Target NameTNF12
• Alternative Names    Tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; TWEAK; APO3 ligand; Tumor necrosis factor ligand superfamily member 12
• Accession No. Swiss-Prot: O43508
  NCBI Gene ID: 8742
• UniprotO43508
• Gene ID407977;8742;
• SDS-PAGE MW 27kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.

Chicheportiche Y., J. Biol. Chem. 272:32401-32410(1997).
Marsters S.A., Curr. Biol. 8:525-528(1998).
Clark H.F., Genome Res. 13:2265-2270(2003).

• Western blot analysis of extracts from COLO cells, HUVEC cells and 293 cells, using TNF12 antibody #33585.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using TNF12 antibody #33585.
• Immunofluorescence analysis of HeLa cells, using TNF12 antibody #33585.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting