• Product NameZEB2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ZEB2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ZEB2.
• Target NameZEB2
• Alternative Names    SIP-1; SIP1; Smad interacting-protein 1; Smad-interacting 1; SMADIP1
• Accession No. Swiss-Prot: O60315
  NCBI Gene ID: 9839
• UniprotO60315
• Gene ID9839;
• SDS-PAGE MW 136kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

Cacheux V., Hum. Mol. Genet. 10:1503-1510(2001).
Wakamatsu N., Nat. Genet. 27:369-370(2001).
Nagase T., DNA Res. 5:31-39(1998).

• Western blot analysis of extracts from HepG2 cells, using ZEB2 antibody #33595.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using ZEB2 antibody #33595.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting