• Product NameDGKI Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous levels of total DGKI protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human DGKI.
• Target NameDGKI
• Alternative Names    DAG kinase iota; DGK-IOTA; DGK-iota; DGKI; EC 2.7.1.107
• Accession No. Swiss-Prot: O75912
  NCBI Gene ID: 9162
• UniprotO75912
• Gene ID9162;
• SDS-PAGE MW 160kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.

Ding L., J. Biol. Chem. 273:32746-32752(1998).
Bowne S.J., Mol. Vis. 6:6-9(2000).

• Western blot analysis of extracts from 293 cells, using DGKI antibody #33619.
• Immunofluorescence analysis of HeLa cells, using DGKI antibody #33619.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting