• Product NameHEXIM1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total HEXIM1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human HEXIM1.
• Target NameHEXIM1
• Alternative Names    Protein HEXIM1; Hexamethylene bis-acetamide-inducible protein 1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Menage a quatre protein 1
• Accession No. Swiss-Prot: O94992
  NCBI Gene ID: 10614
• UniprotO94992
• Gene ID10614;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000

  Immunohistochemistry: 1/100 - 1/300.
  
  Immunofluorescence: 1:100~1:500

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.

Kusuhara M., Biomed. Res. 20:273-279(1999).
Wittmann B.M., Cancer Res. 63:5151-5158(2003).
Ouchida R., Genes Cells 8:95-107(2003).

• Immunofluorescence analysis of HeLa cells, using HEXIM1 antibody #33624.
• Western Blot analysis of RAT-HEART cells using Hexim1 Polyclonal Antibody diluted at 1:2000

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting