• Product NameTFIP8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TFIP8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TFIP8.
• Target NameTFIP8
• Alternative Names    Tumor necrosis factor; alpha-induced protein 8; Head and neck tumor and metastasis-related protein; TNF-induced protein GG2-1; SCC-S2
• Accession No. Swiss-Prot: O95379
  NCBI Gene ID: 25816
• UniprotO95379
• Gene ID25816;
• SDS-PAGE MW 23kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.

Horrevoets A.J.G., Blood 93:3418-3431(1999).
Kumar D., J. Biol. Chem. 275:2973-2978(2000).
You Z., J. Biol. Chem. 276:26398-26404(2001).

• Western blot analysis of extracts from HUVEC cells, using TFIP8 antibody #33631.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TFIP8 antibody #33631.
• Immunofluorescence analysis of A549 cells, using TFIP8 antibody #33631.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting