• Product NamehnRNP C1/C2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total hnRNP C1/C2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human hnRNP C1/C2.
• Target NamehnRNP C1/C2
• Alternative Names    HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2; ROC; hnRNP C1 / hnRNP C2
• Accession No. Swiss-Prot: P07910
  NCBI Gene ID: 3183
• UniprotP07910
• Gene ID3183;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.

Burd C.G., Proc. Natl. Acad. Sci. U.S.A. 86:9788-9792(1989).
Swanson M.S., Mol. Cell. Biol. 7:1731-1739(1987).
Merrill B.M., Nucleic Acids Res. 17:8441-8449(1989).

• Western blot analysis of extracts from HepG2 cells and COLO205 cells, using hnRNP C1/C2 antibody #33658.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using hnRNP C1/C2 antibody #33658.
• Immunofluorescence analysis of HUVEC cells, using hnRNP C1/C2 antibody #33658.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting