• Product NameTOP2A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TOP2A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human TOP2A.
• Target NameTOP2A
• Alternative Names    DNA topoisomerase 2-alpha; EC 5.99.1.3; DNA topoisomerase II; alpha isozyme; TOP2A
• Accession No. Swiss-Prot: P11388
  NCBI Gene ID: 7153
• UniprotP11388
• Gene ID7153;
• SDS-PAGE MW 174kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
Wasserman R.A., Cancer Res. 53:3591-3596(1993).
Lang A.J., Gene 221:255-266(1998).

• Western blot analysis of extracts from HT-29 cells, using TOP2A antibody #33671.
• Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using TOP2A antibody #33671.
• Immunofluorescence analysis of A549 cells, using TOP2A antibody #33671.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting