• Product NameUBF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total UBF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human UBF1.
• Target NameUBF1
• Alternative Names    Autoantigen NOR-90; Nucleolar transcription factor 1; TCFUBF; UBF-1; UBF1
• Accession No. Swiss-Prot: P17480
  NCBI Gene ID: 7343
• UniprotP17480
• Gene ID7343;
• SDS-PAGE MW 90kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

Jantzen H.M., Nature 344:830-836(1990).
Chan E.K.L., J. Exp. Med. 174:1239-1244(1991).
Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).

• Western blot analysis of extracts from HeLa cells and Jurkat cells, using UBF1 antibody #33692.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using UBF1 antibody #33692.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting