• Product NameELAV2/4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total ELAV2/4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ELAV2/4.
• Target NameELAV2/4
• Alternative Names    ELAV-like protein 4; Paraneoplastic encephalomyelitis antigen HuD; Hu-antigen D; ELAVL4; HUD
• Accession No. Swiss-Prot: Q12926/P26378
  NCBI Gene ID: 1993/1996
• UniprotQ12926
• Gene ID1993;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.

Szabo A., Cell 67:325-333(1991).
Behrends U., Int. J. Cancer 100:669-677(2002).
Gregory S.G., Nature 441:315-321(2006).

• Western blot analysis of extracts from HUVEC cells and Jurkat cells, using ELAV2/4 antibody #33728.
• Immunofluorescence analysis of HepG2 cells, using ELAV2/4 antibody #33728.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting