• Product NameActin α-2/3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Actin α-2/3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human Actin α-2/3.
• Target NameActin α-2/3
• Alternative Names    Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2;
• Accession No. Swiss-Prot: P35609/Q08043
  NCBI Gene ID: 88/89
• UniprotP35609
• Gene ID88;
• SDS-PAGE MW 103kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Beggs A.H., J. Biol. Chem. 267:9281-9288(1992).
Tiso N., Biochem. Biophys. Res. Commun. 265:256-259(1999).
Faulkner G., J. Cell Biol. 146:465-475(1999).

• Western blot analysis of extracts from HeLa cells, HepG2 cells and HUVEC cells, using Actin α-2/3 antibody #33759.
• Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Actin α-2/3 antibody #33759.
• Immunofluorescence analysis of HeLa cells, using Actin α-2/3 antibody #33759.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting