• Product NameACVL1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total ACVL1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ACVL1.
• Target NameACVL1
• Alternative Names    ACVL1; ACVRL1; ACVRLK1; ALK-1; Activin receptor-like kinase 1
• Accession No. Swiss-Prot: P37023
  NCBI Gene ID: 94
• UniprotP37023
• Gene ID94;
• SDS-PAGE MW 56kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.

ten Dijke P., Oncogene 8:2879-2887(1993).
Attisano L., Cell 75:671-680(1993).
Berg J.N., Am. J. Hum. Genet. 61:60-67(1997).

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACVL1 antibody #33762.
• Immunofluorescence analysis of HeLa cells, using ACVL1 antibody #33762.
• Western blot analysis of extracts from K562 cells, using ACVL1 antibody #33762.
• Western blot analysis of extracts from 3T3 cells (Lane 2), using ACVL1 antiobdy #33762. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting