• Product NameFRK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total FRK protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human FRK.
• Target NameFRK
• Alternative Names    EC 2.7.1.112; EC 2.7.10.2; Nuclear tyrosine protein kinase RAK; Tyrosine-protein kinase FRK; fyn-related kinase
• Accession No. Swiss-Prot: P42685
  NCBI Gene ID: 2444
• UniprotP42685
• Gene ID2444;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.

Lee J., Gene 138:247-251(1994).
Cance W.G., Cell Growth Differ. 5:1347-1355(1994).
Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005).

• Western blot analysis of extracts from Jurkat cells, using FRK antibody #33770.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FRK antibody #33770.
• Immunofluorescence analysis of HUVEC cells, using FRK antibody #33770.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting