• Product NameKPB1/2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total KPB1/2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human KPB1/2.
• Target NameKPB1/2
• Alternative Names    KPB1; PHKA; Phosphorylase B kinase alpha regulatory chain; skeletal muscle isoform; Phosphorylase kinase alpha M subunit
• Accession No. Swiss-Prot: P46020
  NCBI Gene ID: 5255/5256
• UniprotP46020
• Gene ID5255;
• SDS-PAGE MW 137kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Wuellrich A., J. Biol. Chem. 268:23208-23214(1993).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Burwinkel B., Eur. J. Hum. Genet. 11:516-526(2003).

• Western blot analysis of extracts from Jurkat cells and COLO205 cells, using KPB1/2 antibody #33776.
• Immunofluorescence analysis of HeLa cells, using KPB1/2 antibody #33776.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting