• Product NameCLK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CLK2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CLK2.
• Target NameCLK2
• Alternative Names    CDC-like kinase 2; EC 2.7.12.1; kinase CLK2;
• Accession No. Swiss-Prot: P49760
  NCBI Gene ID: 1196
• UniprotP49760
• Gene ID1196;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Hanes J.J., J. Mol. Biol. 244:665-672(1994).
Winfield S.L., Genome Res. 7:1020-1026(1997).
Lee K., J. Biol. Chem. 271:27299-27303(1996).

• Western blot analysis of extracts from COLO205 cells and A549 cells, using CLK2 antibody #33785.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting