• Product NameCD253 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CD253 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CD253.
• Target NameCD253
• Alternative Names    Tumor necrosis factor ligand superfamily member 10; TNF-related apoptosis-inducing ligand; Protein TRAIL; Apo-2 ligand; Apo-2L
• Accession No. Swiss-Prot: P50591
  NCBI Gene ID: 8743
• UniprotP50591
• Gene ID8743;
• SDS-PAGE MW 30kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Wiley S.R., Immunity 3:673-682(1995).
Pitti R.M., J. Biol. Chem. 271:12687-12690(1996).
Hymowitz S.G., Mol. Cell 4:563-571(1999).

• Western blot analysis of extracts from 293 cells and HUVEC cells, using CD253 antibody #33792.
• Immunofluorescence analysis of A549 cells, using CD253 antibody #33792.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting