• Product NameDGKE Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total DGKE protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DGKE.
• Target NameDGKE
• Alternative Names    Diacylglycerol kinase epsilon; EC 2.7.1.107; Diglyceride kinase epsilon; DGK-epsilon; DAG kinase epsilon
• Accession No. Swiss-Prot: P52429
  NCBI Gene ID: 8526
• UniprotP52429
• Gene ID8526;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.

Tang W., J. Biol. Chem. 271:10237-10241(1996).
Tang W., Gene 239:185-192(1999).
Sjoeblom T., Science 314:268-274(2006).

• Western blot analysis of extracts from K562 cells, using DGKE antibody #33803.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting