• Product NamehnRNP F Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total hnRNP F protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human hnRNP F.
• Target NamehnRNP F
• Alternative Names    HNRPF; ROF; heterogeneous nuclear ribonucleoprotein F;
• Accession No. Swiss-Prot: P52597
  NCBI Gene ID: 3185
• UniprotP52597
• Gene ID3185;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Matunis M.J., Nucleic Acids Res. 22:1059-1067(1994).
Honore B., J. Biol. Chem. 270:28780-28789(1995).
McDonald H., Genomics 13:344-348(1992).

• Western blot analysis of extracts from HepG2 cells, using hnRNP F antibody #33806.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting