• Product NameDGKQ Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DGKQ protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DGKQ.
• Target NameDGKQ
• Alternative Names    DAG kinase theta; DAGK; DAGK4; DAGK7; DGK-theta
• Accession No. Swiss-Prot: P52824
  NCBI Gene ID: 1609
• UniprotP52824
• Gene ID1609;
• SDS-PAGE MW 101kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor By similarity. May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity.

Pilz A., Genomics 26:599-601(1995).

• Western blot analysis of extracts from HT-29 cells, using DGKQ antibody #33812.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting