• Product NameSP3/4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total SP3/4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human SP3/4.
• Target NameSP3/4
• Alternative Names    LAG1 longevity assurance; SP260; TMSG1; Tumor metastasis-suppressor gene 1 protein;
• Accession No. Swiss-Prot: Q02447/Q02446
  NCBI Gene ID: 6670/6671
• UniprotQ02447
• Gene ID6670;
• SDS-PAGE MW 82kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Transcriptional factor that can act as an activator or repressor depending on isoform and/orpost-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.

Pan H., Genomics 77:58-64(2001).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from Jurkat cells, using SP3/4 antibody #33846.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting