• Product NameMED21 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MED21 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MED21.
• Target NameMED21
• Alternative Names    Mediator of RNA polymerase II transcription subunit 21; Mediator complex subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; hSrb7
• Accession No. Swiss-Prot: Q13503
  NCBI Gene ID: 9412
• UniprotQ13503
• Gene ID9412;
• SDS-PAGE MW 16kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Chao D.M., Nature 380:82-85(1996).
Maldonado E., Nature 381:86-89(1996).
Oelgeschlaeger T., Mol. Cell 1:925-931(1998).

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED21 antibody #33869.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting