• Product NameZNF147 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ZNF147 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ZNF147.
• Target NameZNF147
• Alternative Names    EFP; Efp; Estrogen responsive finger protein; TRI25; TRIM25
• Accession No. Swiss-Prot: Q14258
  NCBI Gene ID: 7706
• UniprotQ14258
• Gene ID7706;
• SDS-PAGE MW 71kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Inoue S., Proc. Natl. Acad. Sci. U.S.A. 90:11117-11121(1993).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Rush J., Nat. Biotechnol. 23:94-101(2005).

• Western blot analysis of extracts from K562 cells, using ZNF147 antibody #33878.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting