• Product NameCHD4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total CHD4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CHD4.
• Target NameCHD4
• Alternative Names    EC 3.6.1.-; ATP-dependent helicase CHD4; CHD-4; Mi-2 autoantigen 218 kDa protein; Mi2-beta
• Accession No. Swiss-Prot: Q14839
  NCBI Gene ID: 1108
• UniprotQ14839
• Gene ID1108;
• SDS-PAGE MW 218kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

Seelig H.P., Arthritis Rheum. 38:1389-1399(1995).
Schmidt D.R., Biochemistry 38:14711-14717(1999).
Shimono Y., J. Biol. Chem. 278:51638-51645(2003).

• Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using CHD4 antibody #33885.
• Immunofluorescence analysis of HepG2 cells, using CHD4 antibody #33885.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting