• Product NameTF3B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TF3B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TF3B.
• Target NameTF3B
• Alternative Names    TFIIIB90; hTFIIIB90; B-related factor 1; BRF-1; hBRF
• Accession No. Swiss-Prot: Q92994
  NCBI Gene ID: 2972
• UniprotQ92994
• Gene ID2972;
• SDS-PAGE MW 74kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.

Wang Z., Proc. Natl. Acad. Sci. U.S.A. 92:7026-7030(1995).
Mital R., Mol. Cell. Biol. 16:7031-7042(1996).
McCulloch V., EMBO J. 19:4134-4143(2000).

• Western blot analysis of extracts from HeLa cells and Jurkat cells, using TF3B antibody #34013.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using TF3B antibody #34013.
• Immunofluorescence analysis of MCF-7cells, using TF3B antibody #34013.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting